Plasmid

Part:BBa_K3978041:Design

Designed by: Haozhen He   Group: iGEM21_HiZJU-China   (2021-10-21)


BL21 vector combined with amoA and hao gene sequence to degrade EE2 in wastewater


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1883
    Illegal XbaI site found at 30
    Illegal PstI site found at 2624
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1883
    Illegal PstI site found at 2624
    Illegal NotI site found at 911
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1883
    Illegal BglII site found at 1067
    Illegal BamHI site found at 1318
    Illegal XhoI site found at 2786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1883
    Illegal XbaI site found at 30
    Illegal PstI site found at 2624
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1883
    Illegal XbaI site found at 30
    Illegal PstI site found at 2624
    Illegal NgoMIV site found at 3103
    Illegal NgoMIV site found at 7780
    Illegal AgeI site found at 1342
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400
    Illegal BsaI.rc site found at 1781
    Illegal BsaI.rc site found at 3688
    Illegal SapI.rc site found at 5348


Design Notes

1.you have to use at least two endonuclease to cleave the plasmid for obtain the right orientation of the sequence 2.amoA have 2 subunit, both of which have to be inserted


Source

B0030 RBS; B0015 terminator amoA sequence:BBa_K3978042; hao sequence BBa_K3978043

References